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He scored the winning penalty kick as Madrid beat city rivals Atletico Madrid in a shoot-out to win the Champions League, having scored 16 times throughout the competition.
Ronaldo previously won in 2008, 2013, 2014 with Messi second on each occasion. At EURO 2016, fans of Iceland clapped at the end of the game. The Portuguese gained a series of success with club Real Madrid and the Portugal national team.
The FIFA Best Female Player Award went to World Cup victor and two-time Olympic victor Carli Lloyd, while the FFIFA Puskas Award went to Mohd Faiz Subri, forward, Penang in the 2016 Malaysia Super League. The god of football said Leicester must win!'
Messi stayed away from the bash and Ronaldo finished his acceptance speech by saying he was "sorry some people from Barcelona aren't here, but that's understandable".
Lyod, who won the 2015 FIFA Women's World Player of the Year award, scored 17 goals in 2016 for the USA national team. This achievement is incredible. "It's wonderful and I am very happy".
Voting for the player and coach categories was by national team captains and managers, selected journalists and, for the first time, an online poll of fans.
Instead, the Ireland boss went for Ronaldo's Real Madrid team-mate Gareth Bale as his top pick. Thank you so much for voting for me.
"I've also got to thank my family, my brothers, my mum, my whole staff 100 per cent for everything they've given me". The other nominees were Iceland fans at Euro 2016, and Dutch club ADO Den Haag, whose fans brought soft toys to children supporting rival team Feyenoord.
Elsewhere, the Team of the Year did not feature a single player from the Premier League.
For years now, the Ballon d'Or trophy has been dominated by Ronaldo and Messi.
"It's a great feeling, as I mentioned many times last year was a dream year, no doubt about that", the striker said at the ceremony shortly before receiving the prize. They show I've lived up to the expectations and I achieved the best I could.