The decree issued by the Emir Sheikh Tamim bin Hamad al-Thani amends a 2004 anti-terrorism law, and creates two national terrorism lists which set rules for listing individuals and groups on each list, Reuters reports.
To that, the Emir said tonight that Qatar is "fighting terrorism relentlessly and without compromises, and the worldwide community recognizes this".
Sheikh Tamim said his country was targeted by unprecedented campaign.
"We are open to dialogue to resolve the outstanding problems, so long as Qatar's sovereignty is respected", said the Emir.
The emir added that despite the blockade, life is going along normally in the country.
Qatar refused to bow to the demands within a 10-day deadline, and the anti-Qatar bloc has begun to shift its focus toward six principles on combatting extremism and terrorism. Several other countries then joined the boycott. China had no personal interest in dealing with the Middle East issue, and took the well-being of the countries and people in the region into account.
The order follows an agreement between Qatar and the United States that seeks to curb "terrorism financing", and which was signed during a visit to Doha by US Secretary of State Rex Tillerson. Details have not been released but sources said it provides for the United States to post officials at Qatar's state prosecutor's office.
Qatar has rejected the charges while refusing to accept the demands, citing the right to defend its sovereignty. "The perpetrators have undermined our sovereignty and independence by fabricating false statements to mislead worldwide public opinion".
The UAE this week said it expected a prolonged stalemate in the dispute and gave no hint it would be willing to left the blockade to allow talks to start.