Last month, Bolt jokingly warned Blake there would be "problems" if he failed to win a medal at the games.
His compatriot Henricho Bruintjies came in second clocking 10.17 in the touchline leaving the Jamaican to settle for third at 10.19.
"It's my first worldwide title and for me it's a milestone, a stepping stone towards the world championships, and the Olympics and more competitions and more global competitions", said the 24-year-old. "I just focused on myself".
Blake will feel similarly frustrated and the pre-race favourite said of his ragged performance: "I was stumbling all the way and I didn't recover from it". I knew if I managed to do so, I would get a good result. "That's just shocking to be honest, I should have done better than that".
Bolt, who retired a year ago with eight Olympic golds including two 4x100 relay titles shared with Blake, tweeted his congratulations to his teammate and Simbine.
"Keep putting in the work. You know your journey", the sprint great said.
England's Adam Gemili, the 2014 Commonwealth silver-medallist who was second quickest into the final, withdrew before the race after picking up an injury in the semis.
"I wasn't focused on (Blake)". I just didn't recover from it.
"Being able to run in lane eight and lane nine with Henricho and placing first and second with him, it's really incredible".
There was more disappointment for sprint powerhouses Jamaica as Christania Williams (11.21) and Gayon Evans (11.22) settled for silver and bronze respectively in the women's 100m.
South Africa's Chad le Clos completed a hattrick of butterfly golds when he won the 100m race, and Bronte Campbell earned a rare victory over her sister Cate in the 100m freestyle.
South Africa's Caster Semenya also eased into the 1,500m final after winning her heat. The South Africa flag-bearer, who will run in the 800, qualified fastest for today's final ahead of home runner Georgia Griffith (4:06.41) and Kenya's Mary Kuria.
Even with the absence of Wayde van Niekerk and reigning champion Kirani James, the men's 400 metres boasts quality and looks to be a battle between Botswana's flamboyant Isaac Makwala and Grenada's Rio Olympic finalist Bralon Taplin.
After crossing the line, the 31-year-old did two push-ups on the track to show his fitness and grinned at the TV camera.
New Zealand's world champion and world leader Tom Walsh fulfilled his billing as heavy shot-put favourite with a throw of 21.41m - a metre off his personal best - to claim the title while Uganda's Stella Chesang came through to win an open women's 10,000m final.