Eric Dier, Ruben Loftus-Cheek and Fabian Delph will start in midfield, while Danny Welbeck joins Rashford up front.
Although Kane top-scored at the World Cup with six goals and has two from four games in the Premier League this season, there have been question marks over his overall levels of performance with some pundits suggesting that his drop in shot attempts per game highlights his fatigue.
But if nothing changes he admits he will have to think long and hard about picking those who have been left stuck on the sidelines for next month's Nations League trips to Croatia and Spain.
Southgate insisted after being outplayed for the majority of a 2-1 defeat by Spain in his side's inaugural Nations League encounter on Saturday that England must persist with his intention to improve on playing out from the back under pressure. He's had a lot of attention on him over the summer, over possibly going to other teams but I'm glad he's stayed here.
Gareth Southgate has expressed frustration about Phil Foden's lack of first-team involvement for Manchester City and warned England's senior players they can not expect to keep their places if they are not involved more regularly for their club sides.
Southgate's concerns extend to Foden's restricted time on the pitch for City, with the 18-year-old limited to one substitute appearance, of eight minutes, this season. Everybody has got to play a part in that but the clubs own the player and they have got to do the right thing for themselves.
'It was scruffy at times, but we kept doing it, ' England shot-stopper Butland said. I was at the Manchester City versus Huddersfield game and you think, 'well ok, it is a game where Foden will play'.
"It is not as straightforward for Pep as just, "oh, I'll just give Phil a game".