The roughly 12-minute-long video features global chains such as Hilton Worldwide Holdings-owned Conrad Beijing and Waldorf Astoria, as well as Marriott International's Sheraton and Le Royal Meridien.
The captions indicated that the incident was filmed at the Le Royal Meridien Shanghai, and that he had paid 1,600 yuan ($317) a night for the room. The video has been viewed more than 30 million times.
Huazong, who has 322,000 followers on Weibo, claims he has stayed at 147 five-star and boutique hotels over the past six years.
"But the actual practices on that day did not follow the cleaning standards for room cleaning, we are deeply sorry for this", the hotel apologized in a statement posted to its Weibo account, adding staff would be retrained.
In a 12-minute video posted to the popular Chinese social media site Weibo, cleaning staff were also shown washing cups and mugs with the same sponge used to wipe down the shower and sink.
Most of the affected hotels, which include The Conrad Beijing, Le Royal Meridien Shanghai and The Shangri-La Hotel Fuzhou, are part of large global hotel chains.
A statement issued Thursday by China's Ministry of Culture and Tourism said "all levels of cultural and tourism authorities should draw inferences and give high priority to the supervision of tourism service quality".
The video, which was posted November 14, shows a housekeeper at the Waldorf Astoria Hotel in Shanghai using a single sponge to clean cups and the sink. The hotel apologised for what was shown in the video.
The almost The 12-minute video, posted online earlier this week, shows workers cleaning bathrooms.
A Marriott hotel in Hangzhou in China's Zhejiang province.
"We sincerely apologise for what has occurred and we are now investigating this matter with the highest diligence", said hotel spokeswoman Chloe Qian.
The Shangri-La Hotel in Fuzhou told Sina.com the video was genuine and that its hygiene standards had not been upheld.
The Shanghai Tourism Bureau said they would conduct an investigation and staff members involved will be facing disciplinary action if the video was proven to be genuine, while Beijing Tourism Bureau also said they will talk to the hotels involved and will be sending inspectors to conduct an investigation as well. The Four Seasons in Shanghai said it would improve internal training.