The record-breaking female python the team found contained 73 developing eggs, the preserve reports.
The researchers caught this behemoth by using male pythons equipped with radio transmitters, which, they say, "allows the team to track the male to locate breeding females".
She was captured after officials said pythons had to be rounded-up to stop their numbers getting out of control.
With the capture of pythons, researchers are also able to collect data to develop new removal methods.
They said their teams had been able to remove several other breeding females from the same area in recent months in partnership with the US Geological Survey. The largest one was over 18 feet long and weighed more than 100 pounds, according to the Florida Fish and Wildlife Conservation Commission.
The were brought to the state in the 1970s as pets and can grow up to 20 feet long.
The Burmese python is native to Southeast Asia, but in recent decades the big snakes have become a slithering menace in Florida.
Python hunters are said to have caught more than 1,850 of the snakes in the area after they started to wreak havoc on Florida's ecosystem.
In 2017, 25 hunters were paid to euthanize pythons under a $175,000 pilot program by the South Florida Water Management District. Everglades National Park scientists trained a beagle puppy named Python Pete to sniff out the snakes, but had to abandon the venture when Pete wilted in the heat of the Florida summer.